The enzyme-linked immunosorbent assay (ELISA) is an analytical laboratory technique used to detect and measure the concentration of a substance (usually antibodies or antigens) in solution. The ELISA is being used as adiagnostictool in medicine andplant pathology, as well as aquality controlcheck. The materials used for the test are enzymes, anti-bodies and reagents supplied in a kit form.
|ELISA Kits for||Application|
|Leukotriene- B4/ C4/ D4 and others||Identification and quantification of antigens causing inflammation, allergy etc.|
|5/12/15 Hydroxyeicosatetraenoic acids. (HETE)||Research use for identification of HETEs|
|Lipoxins Research use for identification of||Research use for identification of lipoxins|
|Enzymes of Eicosanoid Cascade||Research and Diagnostics|
Indus Gene will be producing the antibodies and enzymes that are the key components of the Elisa kits. Other components like the wash buffers can be out sourced to assemble the ready to use kit.
ELISAs are typically performed in 96-well (or 384-well) polystyrene plates, which will passively bind antibodies and proteins. It is this binding and immobilization of reagents that makes ELISAs so easy to design and perform. Having the reactants of the ELISA immobilized to the micro plate surface makes it easy to separate bound from non-bound material during the assay. This ability to wash away nonspecifically bound materials makes the ELISA a powerful tool for measuring specific analytes within a crude preparation. A detection enzyme or other tag can be linked directly to the primary antibody or introduced through a secondary antibody that recognizes the primary antibody.
It also can be linked to a protein such as streptavidin if the primary antibody is biotin labeled. The most commonly used enzyme labels are horseradish peroxidase (HRP) and alkaline phosphatase (AP). A large selection of substrates is available for performing the ELISA with an HRP or AP conjugate. The choice of substrate depends upon the required assay sensitivity and the instrumentation available for signal-detection (spectrophotometer, fluorometer or luminometer).